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Transformation - Introducing designed DNA sequences to Arabidopsis thaliana -
How your designed DNA sequences are experimentally introduced into Arabidopsis thaliana?
1. Introduce your designed DNA sequences (useful gene) into a vector for Agrobacterium. (See the details of the vector. )
2. Transform the vector to Agrobacterium.
3. Soak
Arbidopsis thaliana in the suspension of Agrobactrium and leave them for a while.
4. Arabidopsis thaliana cells are infected by Agrobactrium during the process 3.
5. Grow the Arabidopsis and gather seeds.
6. The gathered seeds are mixture of both Agrobactrium-infected and -uninfected seeds. Wild type Arabidopsis is not able to grow in a drug resistance plate, while the Agrobacterium-infected Arabidopsis is able to grow by the action of a drug resistance gene co-existing with the designed DNA sequence in the vector. To select the Agrobacterium-infected seeds, grow the seeds in a drug(hygromycin) resistance plate.