International Rational Genome Design Contest

The above figure of the preceding researches shows that a plant gets a function to eliminate formaldehyde by inserting two enzymes, HPS (3-hexulose-6-phosphate synthase) and PHI (6-phospho-3-hexuloisomerase) into the Calvin Cycle of the plant.

These two protein sequences of PHI, HPS enzymes utilized in the Preceding Research are registered in the project for Swiss-Prot .

HPS: http://scines.org/item/cria214s2ria214u95348i/

PHI: http://scines.org/item/cria214s2ria214u162897i/

For example, if you wish to design DNA sequences by using those data,  refer to the followings.

1. Obtain the HPS protein sequence
2. Reverse-translate the obtained HPS protein sequence  to DNA sequence
3. Obtain PHI protein sequence
4. Reverse-translate the obtained PHI protein sequence to DNA sequence
5. Combine those two DNA sequences
6. Register the combined DNA sequences in RIKEN SciNetS

However, the DNA sequences you have just created may not express in a model plant as you intended to. The following tips might help the DNA sequences perform properly.

• Optimize appearance frequency of codon
• Consider about promoter
• Add signal peptide
• Use fusion proteins
• Use IRES (Internal Ribosomal Entry Site)
• Others

Codon frequencies are registered in Rational Genome Design: Parts collection and Codon Usage Database on RIKEN SciNetS. 

Participants may use non-registered data in SciNeS as long as source of data is duly acknowledged.