We have developed a web-interface software CAD system for designing synthetic plant promoter sequences. Users can start with a baseline or natural promoter sequence, then interface directly with biological databases to alter this DNA sequence using a series of modular data mining functions.
We have uploaded several genomic datasets related to gene expression and promoter sequence to LinkData.org. This semantic web system allows the data to be accessed in a variety of formats, both human and machine readable, and sets up the rows and columns of data files to be easy to hyperlink together or “mashup” to do cross-dataset analysis. We have created 4 such mashups from Arabidopsis thalania databases as a resource for synthetic promoter design.
The creative activity of this challenge is to “mine” the data mashups by looking for new associations and genes with interesting or useful expression pattern. As such, this will require a basic understanding of the data structures and experiments performed (from reading our manuals and descriptions from the database references). Examples can include sets of genes that are all expressed in the same growth condition, at the same time of day, and/or in the same part of the plant. We are providing a basic set of such functions and example workflows which show how to look for a gene expression property (e.g. highly expressed in leaf tissue in young plants) and retrieve the functional DNA sequences to add to a synthetic promoter design.
We will judge submitted functions based on their novelty, usefulness, and implementation. With social networking participants can discuss programming functions, find out which functions the DNA designers with more biological experience would like to have, and even share code under development. We encourage group participation, and our system automatically tracks the history of code forking so credit can be given to all contributors.
If you want to learn more about making your own biological design program, how our data is structured, how to submit and test your program, or anything else about the programming challenge please head over to our manuals section!